Fatty Acid Profiling
The quality and origin of lipid products is examined by gas chromatography of fatty acid methyl esters (FAMEs) derivatised from lipid sources such as triglycerides and phospholipids. FAMEs GC profiles are measured by Area % normalisation. Different fixed oil products are characterised by specific Area % proportions of the various FAMES.
Lipid Compositional Analysis
FAMEs analysis is a rationalisation of the more complex lipids from which component fatty acids are derived. It is sometimes useful to examine the lipid profile in its underivatised form to understand the true composition of a substance. This is achieved by HPLC profiling
Fixed oils comprised of triglycerides, diglycerides and free fatty acids can be derivatised into methyl esters of component fatty acids and profiled by GC. These fingerprints are compared to compendial and in-house specifications for authentication purposes. The same process can also be used for quantitative analysis of individual fatty acids. Beyond this tri- and diglycerides can be analysed by HPLC-MS to ascertain their respective fatty acid composition and chain sequence. Typical services include authentication of vegetable oils and quantitative determination of EPA/DHA in fish oils.